Fine-tuning of gene expression levels is reaching new heights; latest data indicate full control is now possible, even in primary cells

The secret code to full control of gene expression levels is “TET”, Tetracycline-controlled transcriptional activation. In combination with tailor-made adenoviral vector elements you will control and guide expression intensity for better, clearer research results. There are close to no limits concerning cell type; TET works even with primary cells. This applies to knockdown and overexpression systems equally well. TET in combination with the right adenoviral delivery systems allows for stunning results not possible only a few months ago. more.....

Accomplish in-vivo gene modifications with AAV vectors

Receive custom made AAV fast and reliable from an established core facilty

Project oriented: You will overexpress or silence YOUR gene of interest (GOI) using a sophisticated AAV vector platform.

Tissue specific: You will have control over the GOIs expression pattern with

  • a range of AAV serotypes and
  • an array of tissue specific promoters............more.....

90% knockdown rates on GPCR's now easy to achieve

Membrane-spanning GPCRs are notorious for their high resistance against shRNA knockdown applications. Even newest approaches only manage mediocre knockdown rates. Strategies achieving less than 60% knockdown on mRNA levels [1] are still accepted into high impact publications today (Impact factor - 6.5). A scan through recent literature confirms this being the norm rather than the exception [2-3]. more....

Cancer Centers are coming closer to the in vivo situation - Your custom target into your custom cell line in a matter of weeks

Europe's most comprehensive virus vector driven platform for genetic cell modeling will go with you all the way. From custom viral vector cloning to the most sophisticated creation of individually modeled primary or stable cell lines  more....

Core Facilities improve on viral vectors

Institutional Core Facilities are important facilitators of academic research. They ensure high technology standards and form an important source for quick and reliable troubleshooting. With one of the most comprehensive viral vector platforms on the market, SIRION Biotech understands the importance of concentrating specialized technology.  more....

GEN discusses novel ways for nearly perfect gene silencing with RNAi

Jan 2013: Many questions in cell biology can now be answered with powerful gene silencing tools available in a matter of weeks. Off-Target  signatures in the phenotype can be eliminated by knockdown efficiencies far above 80%, which have become a new standard for the modern laboratory. GEN in its December edition explains how.  more....

Most efficient inducible gene expression with latest All-in ONE vector technology

Nov 2012: Cells adapt to genetic modifications which can lead in certain cases to the selection of mutants escaping the genetic phenotype. Considering the adaptive capacity of cells, inducible gene expression is a valuable tool for functional gene analysis.

SIRION`s 3rd generation inducible platform offers: more....

Maximize your Adenovirus uptake in murine cells….and profit from up to 50-fold enhanced gene expression with AdenoBOOST reagent

Nov 2012: Adenovirus transduction can be challenging: specifically when cells express little CAR (CoxsackieAdenovirus Receptor), key to infect ells. SIRION Biotech`s AdenoBOOST reagent is a non-toxic peptide based solution bridging the adenovirus to the cell surface. Results are phenomenal. more...

All-In ONE vectors for most efficient homogeneous inducible gene expression: near quantitative inducible knockdowns with latest lentivirus technology and RNAiONE

Oct 2012: Meaningful shRNA experiments go beyond validated knockdowns in excess of 90%. They include appropriate controls like confirming on-target phenotypes. Latest All-in ONE lentivirus technology allows for even more precise results when combining it with RNAiONE (3rd generation).

more....

Journal of Gene Medicine reports on improvements with lentivirus infection: novel adjuvants allow for up to 3 fold transduction enhancement

Aug 2012: Lentivirus remains the most favored viral vector system for stable gene transfer into mammalian cells. Its activation of stress signal pathways is minimal as are other phenotypic alterations. Lentiviral transduction efficiency, though, remains a hurdle when applying lentivirus to a broad range of cell types.

more....

Inducible Knockdowns of nearly 100%!? – Is that possible? Ask for your Money Back Gurantee

Munich's CellCompetence Team is celebrating its first application of a nearly 100% inducible knockdown. What was considered impossible only a few years ago, is now becoming an industry standard. This gives reason to a first Money Back Gurantee in the industry.

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Distinguish cancer from stromal cells in co-culture for your drug-testing applications

Fluorescent 3D tumor co-culture systems allow for mimicking the typical xenograft microenvironment more closely. Latest technology enables the scientist to easily distinguish cancer from stromal cells in co-culture. One is expressing GFP, the other RFP. All applicable on standard plate readers.

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First time ever: Customer Satisfaction Guaranty for your Gene Knockdowns 

Over the years SIRION Biotech has developed its base technology into an art: in 80% of recent projects SIRION Biotech managed to identify shRNAs with knockdown results of above 80%. These results enable us to offer a Customer Satisfaction Guaranty. Project statistics below demonstrate how prevalidation results translate fully into KDs in cell product.

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VariCHECK - switch most easily between wild type and mutant expression

Novel technology lets you massively improve on your mutation analysis:

  • one step functional analyses of any protein variant
  • for more sophisticated cancer resistance models
  • for better protein aggregation assays
  • for modern cell based models for neurodegenerative diseases

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SenCELLTM - in vitro cell models with built in reporter genes for more effective screening

SIRION Biotech this March launched its new line of products for functionality testing in stable in vitro cell systems. Take e.g.
SenCELL LifeAct TM representing a range of First in Class Sensor Cell Lines

  • sensor molecules that identify actin dynamics
  • no interference with dynamic processes during cytoskeleton rearrangements

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Adenovirus vector cloning & production in less than 4 weeks; purification in only 20 minutes!

Gene delivery is now made easy and affordable to every lab. Munich's CellCompetence Center has just made available two brand new kits under its AdenoONE label: 

  • The AdenoONETM Cloning Kit enables recombination based adenoviral vector generation in E.coli cells followed by production in HEK 293 cells, within less than 4 weeks. Adenovirus particles are generated from an E1/E3 deleted adenoviral serotype 5 and therefore comply with required safety standards.

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Inducible Knockdowns of nearly 100%!? – Is that possible?

The CellCompetence Team is celebrating its first application of a nearly 100% inducible knockdown. What was considered impossible only a few years ago, is now becoming an industry standard. 

Example: Maximizing inducible gene knockdown by single cell cloning

Conditional gene silencing is what the researcher wishes to show. Towards that goal, limited dilution helped generating clonal cell lines from the stable pool. Subsequent quantification of target gene X in 25 Dox-treated clones then showed significant variations in mRNA expression levels.

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When it is your profession to design cell models for improved in vitro and in vivo cell applications, you may be interested to know of Europe's leading 

Cell Competence Center®

where colleagues of yours are managing knockdowns of over 90% and turn cDNA into cell models within 6 weeks only. 

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