Adenovirus vector cloning & production in less than 4 weeks; purification in only 20 minutes!

Gene delivery is now made easy and affordable to every lab. Munich's CellCompetence Center has just made available two brand new kits under its AdenoONE label: 

  • The AdenoONETM Cloning Kit enables recombination based adenoviral vector generation in E.coli cells followed by production in HEK 293 cells, within less than 4 weeks. Adenovirus particles are generated from an E1/E3 deleted adenoviral serotype 5 and therefore comply with required safety standards.

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Inducible Knockdowns of nearly 100%!? – Is that possible?

The CellCompetence Team is celebrating its first application of a nearly 100% inducible knockdown. What was considered impossible only a few years ago, is now becoming an industry standard. 

Example: Maximizing inducible gene knockdown by single cell cloning

Conditional gene silencing is what the researcher wishes to show. Towards that goal, limited dilution helped generating clonal cell lines from the stable pool. Subsequent quantification of target gene X in 25 Dox-treated clones then showed significant variations in mRNA expression levels.

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When it is your profession to design cell models for improved in vitro and in vivo cell applications, you may be interested to know of Europe's leading 

Cell Competence Center®

where colleagues of yours are managing knockdowns of over 90% and turn cDNA into cell models within 6 weeks only. 

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