Stably overexpressing cell lines

 

Receive a custom made cell line stably expressing... 

Service Description

- Cloning the cDNA of Your Gene of Interest (GOI) in an expression vector

- Verification of cloning success by DNA-Sequencing

- Transfection/transduction of Your Cell Line with the resulting expression vector

- Generation of stable cell pools or clonal cell lines by antibiotic selection.

- Quantification of  expression of your GOI by qRT-PCR and/or Western Blot

 

Benefits

- Maximum flexibility in experimental design

- Homogenous GOI expression both on mRNA and protein level.

- GOI Expression adjustable by choice of promoter

- From receipt of your cDNA to delivery of a functionality tested stable cell pool in less than 6 weeks

- Funcionality tested frozen cells ready to use

 

Available Expression Systems

 - Monocistronic or bicistronic expression of your GOI

 - In all bicistronic vectors the expression of your GOI is coupled to that of the gene confering antibiotic resistance

Vector Promoter GOI expression Resistence Vector type
CMV-LV CMV monocistronic Puro or Neo lentivirus
CMV-P CMV monocistronic Puro or Neo plasmid
EF1a-LV human EF1a bicistronic Puro or Neo lentivirus
EF1a-P human EF1a bicistronic Puro or Neo plasmid
Ubiqc-LV human Ubiq  c bicistronic Puro or Neo lentivirus
Ubiqc-P human Ubiq  c bicistronic Puro or Neo plasmid

Price for service is based on the customized options chosen and is available on request

For further information or to order please click here or contact a representative of our sales team