Stable knockdown cell lines

Receive a custom made cell line expressing shRNA against your gene of interest )GOI). 

Service Comprises

  • Cloning of validated shRNA into an expression vector
  • Verification of cloning success by DNA-Sequencing
  • Transfection/transduction of Your Cell Line with the resulting expression vector
  • Generation of stable cell pools or clonal cell lines by antibiotic selection
  • Quantification of gene knockdown by qRT-PCR
  • Control cells expressing a Non-Target (NT) shRNA are included

Benefits

  • Reliable system for highest target gene knockdown
  • Guaranteed knockdown of >80% on mRNA level
  • From shRNA sequence to delivery of a functionality tested stable knockdown cell pool in less than 6 weeks
  • Funcionality tested frozen cells ready to use

 

Available Expression Systems
RNA Polymerase III driven  expression of target gene-specific shRNA from human U6 promoter

Vector System Promoter transgene Vector type
U6-shRNA-Puro-LV human U6 shRNA lentiviral
U6-shRNA-Puro-P human U6 shRNA plasmid
U6-shRNA-Neo-LV human U6 shRNA lentiviral
U6-shRNA-Neo-P human U6 shRNA plasmid

 

Price for service is based on the customized options chosen and is available on request

For further information or to order please click here or contact a representative of our sales team