TET-inducible knockdown cell lines

Receive a inducible, custom made knockdown cell line against your gene of interest (GOI). 

Service Comprises

  • Cloning of validated shRNA into the One-Vector inducible shmir platform
  • Verification of cloning success by DNA-Sequencing
  • Transfection/transduction of Your Cell Line with the resulting expression vector
  • Generation of stable cell pools or clonal cell lines by antibiotic selection
  • Quantification of inducible gene knockdown by qRT-PCR
  • Control cells expressing a Non-Target (NT) shRNA are included

 

Benefits

  • Tight system with extremly low background expression in the absence of Dox
  • Strong induction of GOI expression in the presence of Dox both on mRNA and protein level.
  • From receipt of your cDNA to delivery of a functionality tested stable cell pool in less than 6 weeks
  • Funcionality tested frozen cells ready to use

Available Expression Systems

One-Vector systems:
Target gene-specific shRNA (RNA Polymerase II driven) and  TET-responsive  transactivator expressed from the same vector

Vector System Type of regulation transgene Vector type
One-vector-LV   TET-ON shRNA-mir lentiviral
One-vector-P   TET-ON shRNA-mir plasmid

Price for service is based on the customized options chosen and is available on request

For further information or to order please click here or contact a representative of our sales team