Custom Knockdown Adenovirus vector

Receive a custom made Adenovirus to knockdown your gene of interest (GOI).

Service Comprises

  • shRNA validation based on 10 or 15 sequences
  • Cloning of validated shRNA(s) into adenovirus shuttle plasmids
  • Verification of cloning success by DNA-Sequencing
  • Generation of recombinant adenovirus BACs
  • Production of low-passage virus stocks
  • Amplification of the adenovirus vector in 293 cells
  • Purification and Titration of infectious units (IU)
  • Gene expression quantification on mRNA level by qRT-PCR

Benefits

  • Patent pending BAC technology guarantees fast time lines resulting in less than 6 weeks from cloning to final virus preparation
  • Highest product purity
  • Quantitative shRNA delivery to and uniform expression
  • Functionality tested and guaranteed gene knockdown ≥ 80% on mRNA level
  • Degree of Knockdown adjustable by using different virus amounts
  • Reproducible results by high efficient titration

Available Expression Systems
Different available promotors and marker genes guarantee for flexibility in vector design

ScaleInfectious Units (IU)TimePrice
Standard ≥ 1 X10E94-5 weeks3.100,00 EUR + shRNA validation
Medium≥ 1 X10E105-6 weekson request
Large≥ 1 X10E117-8 weekson request
X-Large≥ 1 X10E128-10 weekson request

For further information or to order please click here or contact a representative of our sales team