MISSION shRNA cells
Your fast lane to stable knock down cell lines
The generation of knock down cell lines is laborious and often takes months before getting first results. By offering cell pools of lentiviral transduced cell lines SIRION BIOTECH and Sigma Aldrich offer a quick, easy and convenient way to get your individual stable knock down cell line. As the cells are tested to be virus free no extra precautions for handling with lentivirus particles must be taken. The cell pools are produced by using the high quality shRNA lentivirus libraries MISSION TRC-1 or TRC-2 from Sigma Aldrich. A significant synergy between the world’s largest library from Sigma Aldrich and SIRION BIOTECH’s years of experience in customized cell line generation.
Your advantage:
- virus-free handling
- no adaption of lentivirus technology necessary
- optimized protocols for cell pool generation
- fast processing and reliable results
- genome wide coverage with more than 30.000 human and murine genes
- customized for your cell line
By default 4 to 5 TRC clones of the gene of interest are used for transduction. For each TRC clone one stable cell pool is established by puromycin selection giving 4 to 5 cell pools per gene. The customer can send his own cells to Sigmas’ Service or choose cells from ECACC culture collection.
Proof of gene knock down for best choice of clones
Figure A
A: Established Cos-1 MISSION shRNA cell pools are shown here in transmitted light microscopy in comparison to parental control and non target shRNA control. Before cells were transduced with lentivirus particles expressing shRNA against hCyp26A1 and submitted to a 3 weeks puromycin selection.
Figure B
B: The cell pools were analyzed for gene expression by using qRT-PCR and were compared to parental control cells and non-target shRNA control cells showing two cell pools with more than 90% knock down.
Additional services / Optional
Supply of homogenious, stable, validated clonal cell lines for efficient high thoughput screens
Depending on the customers demand we also generate defined clones by serial dilution and subsequent clonal selection from the polyclonal stable cell pools.
