Instead of relying on algorithms that might work SIRION BIOTECH offers a validation service which guarantees you information about the biological activity of shRNA sequences.

Our shRNA selection technology allows fast and reliable shRNA validation in vitro, leading immediately to shRNA sequences with unmatched gene silencing efficacy. Whenever efficiency is an issue, SIRION BIOTECH can identify the most potent shRNA sequence for your gene silencing assay.

The transcript or gene of interest is cloned into SIRION BIOTECH's unique validation vector system along with the co-expressed shRNA. Knockdown efficiency is then validated by light cycler qPCR. shRNA sequences designed and validated by SIRION BIOTECH are a must for

difficult cellular models
viral transcripts
non-coding RNA transcripts
generation of stable knockdown cell lines
in vivo applications
extreme high KD efficiencies >95%

Application example: Identification of potent shRNA sequences against a bovine stem cell target PLAC1 (figure 1).

Figure1:
Screening of shRNAs against a bovine stem cell-expressed protein yielded 3 sequences with >85% activity on mRNA.

Sales Contact

For prices and further information please inquire:

T. +49 89 700 96 19 99
info@sirion-biotech.de