Adenoviral vectors are well-established gene transfer tools for quantitative gene transfer into mammalian cells. Adenoviral vectors enter cells after binding to cellular receptors by using the cell’s endocytosis pathway [Reference1, 2]. SIRION BIOTECH’s adenoviral vector platform allows for high efficient knock down as well as over-expression of genes even in hard-to-transfect cell types, including primary cells. The specificity of adenoviral vector transduction can be modified to target specific cell types or sub-populations [Ref.3]. SIRION BIOTECH is able to generate adenovirus libraries of any size for functional gene analysis by exploiting high-efficient recombination in vivo.

Advantages:

High virus titer
High purity
Long lasting gene knock down
Accessibility to hard-to-transfect cell lines and primary cells
Certified gene knock down >80%

Expertise:

SIRION BIOTECH offers virus-treated cells as Q-tech frozen cell stocks which are transiently expressing the gene of interest in almost all human cell lines and in primary cells. Quantitative transduction of mammalian cells across species can be achieved with adenoviral vectors (Figure 1). Accordingly, highly efficient gene knock down in different cell types including primary cells can be achieved (Figure 2). SIRION BIOTECH offers Q-tech cell products with pre-established knock down certified for >80% knock-down on protein level. SIRION BIOTECH’s adenoviral production service guaranties high purity adenovirus preparations up to 1012 infectious particles with certified gene expression in your cells of choice.

Figure1: Expression of GFP in primary myoblast from different species transduced with an adenoviral vector expressing GFP.

Figure 2: Western Blot demonstrating the knock down of P53 and pRb in human A549 cell line and CHIP protein in primary human myoblasts.

Safety:

SIRION BIOTECH’s adenoviral vectors are safe and can only be replicated in special production cell lines. All preparations are tested for the absence of replication competent viruses.

Adenovirus Technology References:

Wickham TJ, Mathias P, Cheresh DA, Nemerow GR. Integrins alpha v beta 3 and alpha v beta 5 promote adenovirus internalization but not virus attachment. Cell. 1993 ; 73:309-19.
Bergelson JM, et al. Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5. Science. 1997; 275:1320–1323.
Volpers C, Thirion C, Biermann V, Hussmann S, Kewes H, Dunant P, von der Mark H, Herrmann A, Kochanek S, Lochmüller H. Antibody-mediated targeting of an adenovirus vector modified to contain a synthetic immunoglobulin g-binding domain in the capsid. J. Virol. 2003; 77:2093-104.

Adenovirus Services

Custom Adenovirus production here

Sales Contact

For prices and further information please inquire:

T. +49 89 700 96 19 99
info@sirion-biotech.de