Profit from 20 to 50-fold enhanced adenovirus transduction using SIRION BIOTECH`s enhancer solution for adenovirus uptake!

Recombinant adenoviruses possess the tropism of the parent viruses, which can infect all cells possessing the appropriate primary adenovirus receptor CAR (Coxsackie Adenovirus Receptor). However some cell types express only low or even none levels of CAR, making efficient recombinant adenovirus transduction almost impossible.

AdenoBOOST^TM specifically bridges the adenovirus surface to the cell membrane, thereby enhancing gene expression by 20 to 50-fold into cell types which lack the primary adenovirus receptor CAR. 

Product Description:

AdenoBOOST^TM is a non-toxic peptide-based solution

• simply added to the adenovirus prior transduction
• specifically bridging the adenovirus surface to the cell membrane
•  increasing the binding of adenovirus to cell surface

Benefits

• 20 to 50-fold enhanced expression
• Access to nearly all cell types
• Reduced adenovirus amounts
• No cell toxicity

Available:

Application Examples

A set of mammalian and rodent cell lines were transduced with an adenovirus Serotype 5 expressing EGFP with a MOI of 200 or 100 without and with preincubation of AdenoBOOST^TM. For each cell line tested, AdenoBOOST^TM significantly enhanced EGFP expression.