Adenoviral Gene Expression Cells

Fields of Application

Optimal for non-dividing cells or cells with limited doubling capacity such as many Primary Cells
For studying short-term effects (up to 10 days) mediated by the expressed transgene
If transgene expression has adverse effects on cell growth and/or vitality
For screening

Service Comprises

Transduction of Your Cells with an adenoviral expression vector for Your Gene of Interest (GOI)
Freezing of cells 48h after transduction
Quantification of gene expression by qRT-PCR and/or Western Blot relative to control cells
Empty vector transduced control cells are included

Benefits

Quantitative gene delivery to and uniform expression in most cell types
Highest gene expression levels
Gene expression adjustable over a wide range by virus amount (copy number)
Cells tested to be virus-free allowing handling in S1 laboratories
From cDNA sequence to delivery of functionality tested gene expression cells in less than 6 weeks
Funcionality tested frozen cells ready to use

Available Expression Systems

Vector System	Promoter	Transgene(s)	Vector type
Ad-CMV	CMV	cDNA up to 7,5 kb	adenoviral
Ad-EF1a	EF1a	cDNA up to 7,5 kb	adenoviral

Other promoters including those provided by the customer upon request.

Price for service is based on the customized options chosen and is available on request.

Application Example

Transient expression of gene X in U87MG cells transduced with the Ad-CMV-TargetX vector. Western Blot showing gene X expression on days 3, 4 and 5 after transduction relative to cells transduced with an empty control vector (Ad-ctrl).