Adenoviral Gene Knockdown Cells

Fields of Application

Optimal for non-dividing cells or cells with limited doubling capacity such as many Primary Cells
For studying knockdown-mediated gene function (KD stable for up to 10 days)
If target gene knockdown has adverse effects on cell growth and/or vitality

Service Comprises

Transduction of Your Cells with a validated adenoviral shRNA vector
Freezing of cells 48h after transduction
Quantification of gene knockdown by qRT-PCR and/or Western Blot relative to control cells
Control cells expressing a Non-Target (NT) shRNA are included

Benefits

Quantitative shRNA delivery to and uniform expression in most cell types
Highest possible target gene knockdown
Degree of Knockdown adjustable over a wide range by virus amount
Minimum guaranteed kockdown of 80% on mRNA
Cells tested to be virus-free allowing handling in S1 laboratories
From shRNA sequence to delivery of functionality tested knockdown cells in less than 6 weeks
Funcionality tested frozen cells ready to use

Available Expression Systems

RNA Polymerase III driven expression of target gene-specific shRNA from human U6 promoter. Optional: Coexpression of GFP

Vector System	Promoter	Transgene(s)	Vector type
Ad-U6-shRNA	human U6	shRNA	adenoviral
Ad-U6-shRNA-GFP	human U6	shRNA +GFP	adenoviral

Price for service is based on the customized options chosen and is available on request

Application Example

Transient knockdown of CDK5 in HUVEC cells transduced with the Ad-U6-shRNA vector. Western Blot showing CDK5 expression on days 4, 6 and 8 after transduction relative to NT-shRNA expressing control cells.