Fields of Application

• Optimal for non-dividing cells or cells with limited doubling capacity such as many Primary Cells
• For studying knockdown-mediated gene function (KD stable for up to 10 days)
• If target gene knockdown has adverse effects on cell growth and/or vitality

Service Comprises

• Transduction of Your Cells with a validated adenoviral shRNA vector
• Freezing of cells 48h after transduction
• Quantification of gene knockdown by qRT-PCR and/or Western Blot relative to control cells
• Control cells expressing a Non-Target (NT) shRNA are included

Benefits

• Quantitative shRNA delivery to and uniform expression in most cell types
• Highest possible target gene knockdown
• Degree of Knockdown adjustable over a wide range by virus amount
• Minimum guaranteed kockdown of 80% on mRNA
• Cells tested to be virus-free allowing handling in S1 laboratories
• From shRNA sequence to delivery of  functionality tested knockdown cells in less than 6 weeks
• Funcionality tested frozen cells ready to use

Available Expression Systems

RNA Polymerase III driven expression of target gene-specific shRNA from human U6 promoter. Optional: Coexpression of GFP

Price for service is based on the customized options chosen and is available on request