Fields of Application

• For specific and efficient transgene delivery and expression in vivo

Service Comprises

• Cloning of the cDNA of your gene of interest (up to 7.5 kb) /shRNA in an adenovirus shuttle plasmid
• Verification of cloning success by DNA-Sequencing
• Generation of recombinant adenovirus BACs
• Production of low-passage virus stocks
• Amplification in 293 cells
• Purification and Titration of infectious units (IU)
• Gene expression quantification on mRNA level by qRT-PCR and/ or optionally on protein level by Western Blot in a test cell line

Benefits

• Patent pending BAC technology guarantees fast time lines
• Highest product purity
• Degree of overexpression/ knock down adjustable by using different virus amounts
• Reproducible results due to standardized product quality
• Vector formulation for direct use in vivo

Available Expression Systems

Different available promotors and marker genes guarantee for flexibility in vector design