Fields of Application

• For strong, transient gene overexpression in vitro and in vivo

Service Comprises

• Cloning of the cDNA of your gene of interest (up to 7.5 kb) in an adenovirus shuttle plasmid
• Verification of cloning success by DNA-Sequencing
• Generation of recombinant adenovirus BACs
• Production of low-passage virus stocks
• Amplification on 293 cells
• Purification and Titration of infectious units (IU)
• Gene expression quantification on mRNA level by qRT-PCR and/ or
• optionally on protein level by Western Blot in a test cell line (usually 293T)

Benefits

• Patent pending BAC technology guarantees fast time lines resulting in less than 6 weeks from cloning to final virus preparation
• Fast and reliable time lines
• Highest product purity
• Degree of overexpression adjustable by using different virus amounts
• Reproducible results by high efficient titration

Available Expression Systems

Different available promotors and marker genes guarantee for flexibility in vector design