Clonal Cell Line Service

Service Comprises

Transfection/transduction of Your Cell Line with an expression vector of your choice
Generation of stable cell pools by antibiotic selection.
Establishment of single cell clones using the Genetix Clone select Imager
Small scale expansion of individual clones
Identification of "High Performers" via quantification of target gene or GOI expression by qRT-PCR and/or Western Blot relative to control cells
Large scale expansion and cell banking of selected clones
Final QC: Transgene expression, growth characteristics, long-term cultivation (20 passages)
optional: marker gene expression profiling, genotyping, genomic mapping of transgene integration site

Benefits

Clonal cell lines with optimal transgene expression
Guaranteed stability of cell clones
Cell banking of individual cell clones for high inter assay stability
Frozen cells ready-to-use

Available service

Clonal Gene Knockdown cell lines
Clonal Gene Overexpression cell lines
Clonal Reporter Gene cell lines (e.g. luciferase, fluorescent reporter)
Clonal FRT cell lines for isogenic transgene expression
Clonal cell lines for TET ON/TET-OFF inducible gene expression

Application Example

The Caco-2 cells are used across the pharmaceutical industry as an in vitro model of the human small intestinal mucosa to predict the absorption of orally administered drugs. In order to understand mechanisms of drug efflux better, Sirion generated a Caco-2 cell line with stable knockdown of P-Glycoprotein 1 (pgp1) >80% for 20 passages.

Clone4 of Caco-2 cells with pgp-1 knockdown grow to functional monolayers with intact barrier function.