SALES CONTACT

For prices and further information please inquire:

T. +49 89 700 961 99 13
info@sirion-biotech.de
 

Conditional Primary Cell Immortalization

Service Comprises

  • Primary cell isolation from tissue
  • Establishment and optimization of  cell culture conditions
  • Development of immortalization strategies
  • Selection of immortalized cell lines
  • Characterization of conditionally immortalized cell clones

Benefits

  • Controlable expression of the immortalizing gene due to contional immortalization
  • Differentiation of conditionally immortalized cells is possible
  • Makes primary cells available to primary screening
  • Endogenous cell models for drug research and screening
  • Limited expansion maintains primary cell properties
  • Cell banking guarantees homogenous cell quality
  • Reliable results in drug research and screening

Available service for human cell types

  • Human Umbilical Vein Endothelial cells (HUVEC)
  • Human Bronchial Epithelial Cells
  • Human Normal Dermal Fibroblasts
  • Other cell types on request

Service

Product

Primary cell Immortalization

Cat.-No.:

Price for service is based on the customized options chosen and is available on request.

Application Example


Experimental Characterization:


HUVEC Passage 2

T58A HUVEC Passage 15

Morphology
VE Cadherin

+++

++

VEGFR2

+++

+++

TNF Signalling:
ICAM, E-selectin, VCAM-1

+++

+++/++

Tube formation

+++

++

LDL uptake

+++

+++