in cooperation with:

SALES CONTACT

For prices and further information please inquire:

T. +49 89 700 961 99 13
info@sirion-biotech.de
 

MISSION Cells

Fields of Application

  • Functional gene analysis
  • Target identification and validation
  • Pathway analysis and functional screening

Service Comprises

  • Purchase of a set of five different lentiviral transduction particles from the MISSION shRNA library from Sigma targeting Your Gene of Interest (GOI)
  • Transduction of Your Cell Line with the lentiviral vectors
  • Generation of five stable cell pools or clonal cell lines by antibiotic selection.
  • Quantification of gene knockdown by qRT-PCR for each pool  relative to control cells
  • Control cells expressing a Non-Target (NT) shRNA are included

Benefits

  • Genome-wide coverage with more than 30.000 human and murine genes
  • Customized for your cell system
  • Validated stable knockdown cell pools in 6 weeks
  • Cells tested to be virus-free allowing handling in S1 laboratories
  • Funcionality tested frozen cells ready to use

Vector System

Number of TRC clones

Number of cell pools

Price

MiSSION shRNA library

 5

5 + 1 control

6950 €

Application Example

A: Established Cos-1 MISSION shRNA cell pools are shown here in transmitted light microscopy in comparison to parental control and non target shRNA control. Before cells were transduced with lentivirus particles expressing shRNA against hCyp26A1 and submitted to a 3 weeks puromycin selection.

B: The cell pools were analyzed for gene expression by using qRT-PCR and were compared to parental control cells and non-target shRNA control cells showing two cell pools with more than 90% knock down.