Whenever a more predictive model than 2D is necessary

Service Comprises

• Initial test for 3D microtissue formation
• Transfection/transduction of your cell line with an expression vector
• Generation of  genetically modified cell pools
• Control 3D microtissues are included
• Production of 3D microtissues using the InSphero technology
• Quantification of target gene or transgene expression by qRT-PCR and/or Western Blot relative to control cells
• Includes size and culture-medium selection as well as microscopic analysis. Customer receives two 96-well plates of assay-ready microtissues and a report

Benefits

• Ready-to-assay. From shRNA sequence to delivery of a functionality tested stable knockdown 3D microtissues in less than 10 weeks
• Higher Predictivity. 3D genetically modified microtisues will give you more relevant results than using 2D cell culture techniques.
• Co-Cultures are possible: To investigate biological effects co-cultures can be produced, e.g. tumor cell lines with 3T3 Fibroblasts or primary stroma cells.

Available 3D Microtissues

Please specify your cell line and target of interest

Price for service is based on the customized options chosen and is available on request.