The most promising options for in vivo application of viral vectors.
In vivo expression and knockdown experiments help uncover the phenotypic impact of a genetic modification on the level of the living organism.
The biological characteristics of adeno-associated viruses (AAVs) make them ideal for in vivo application of viral vectors.
Benfits of AAV:
- Lack of apparent pathogenicity → low immunogenicity
- Long term gene expression in vivo(> 1 year)
- Infects dividing and non-dividing cells
- Different serotypes/ promoters allow for highest tissue specificity
- BL-1 (S1) compatible in Germany (other safety regulations might apply in other countries)
SIRION BIOTECH specialize in custom adeno-associated virus services for design and AAV production of serotypes AAV1-6 and 1.2 in less than 8 weeks. The AAV virus production service allows for the integration of virtually any desired expression construct within the vector's cloning capacity (4.5 kb). Highly purified and concentrated AAVs enable non-toxic in vivo delivery.
SIRION BIOTECH offers a range of several different AAV serotypes in combination with tissue specific promotors to further increase gene expression precision. Additional in-house features of the SIRION BIOTECH adeno-associated virus technology platform include surface modified particles for keratinocyte (KeraAAV) specific transduction.Any project is thoroughly discussed with our customers to ensure that the resulting particles are feasably designed and fit the final application.