CUSTOM CONSTITUTIVE

Fields of Application

  • Strong, tightly regulated and highly efficient transient gene overexpression and knockdown in vitro
  • for primary cells and all hard to transfect cell types
  • High titer production for in vivo applications

SIRION`s benefits

  • Up to 4 genes from one vector through large packaging capacity (up to 7.5 kb)
  • Large promoter and marker gene portfolio for max flexibility in vector design
  • From cloning to viral delivery in less than 5 weeks – guaranteed with patented BAC technology
  • Highest product purity
  • Reproducible results through high-efficiency titration
  • Tight control of gene expression levels by adapting virus concentration (MOI)
  • knockdown efficiency of at least 80% on mRNA level when combining with our RNAiONE service

Service Details

  • Cloning of customer cDNA (up to 7.5 kb)/ shRNA in SIRION`s adenovirus shuttle plasmid
  • Verification of cloning success by DNA sequencing
  • Generation of recombinant adenovirus BACs
  • Production of low-passage virus stocks followed by amplification in 293 cells
  • Purification, titration of infectious units (IU) and QC
  • Gene expression quantification on mRNA level by qRT-PCR in a standard cell system and/ or
  • optionally on protein level by Western Blot
  • optionally transient cell model generation


ScaleInfectious Units (IU)Time
Standard
≥ 1 X10E9
4-5 weeks
Medium
≥ 1 X10E10
5-6 weeks
Large *
≥ 1 X10E11
7-9 weeks
X-Large*
≥ 1 X10E12
9-11 weeks

* Feasibility check needed/ also applicable forin vivo