Field of Application

  • stable cell model generation to analyze long term effects of gene overexpression and silencing in vitro
  • for dividing and non-dividing cells

SIRION`s benefits

  • optimized lentiviral vector design for highly homogeneous cell model generation
  • custom lentivirus in less than 4 weeks
  • variety of different mono and bicistronic viral vector backbones
  • large promoter and marker gene portfolio guarantees for flexibility in vector design
  • knockdown efficiency of at least 80% on mRNA level when combining with our RNAiONE service

Service Details

  • Cloning of customer cDNA/ shRNA in SIRION`s lentiviral expression vector
  • Verification of cloning success by DNA-Sequencing
  • Lentivirus production in Hek 293 cells
  • Purification, concentration and QC
  • Lentivirus titration by qRT-PCR [VP/ml]
  • optional PFU [IU/ml]
  • optional: SIRION offers full cell model generation service
Standard scales Titer in Viral Particles (VP) Time
Standard ≥ 1 X10E9 2-4 weeks
Medium ≥ 1 X10E10 3-5 weeks
Please contact us for more information