CUSTOM INDUCIBLE

SIRION Biotech`s inducible expression platform is based on the latest 3G TET technology. This guarantees a highly sensitive doxycycline-inducible system without leakiness. Our inducible adenovirus platform is based on a TWO vector system.


Fields of Application

  • Characterization of toxic gene modulations
  • Strong, tightly regulated inducible gene overexpression in vitro
  • Highly efficient inducible gene knockdown studies in vitro
  • for primary cells and all hard to transfect cell types

SIRION`s benefits

  • tightly controlled ON/ OFF switch by doxycycline treatment
  • From cloning to viral delivery in less than 5 weeks – guaranteed with patented BAC technology
  • Highest product purity
  • Reproducible results by high efficient titration
  • Tight regulation of gene expression levels with different MOIs
  • Large promoter and marker gene portfolio guarantees for flexibility in vector design
  • knockdown efficiency of at least 80% on mRNA level when combining with our RNAiONE service

Service Details

  • Cloning of your cDNA (up to 6 kb)/ shRNA in SIRION`s inducible adenovirus shuttle plasmid
  • Verification of cloning success by DNA sequencing
  • Generation of recombinant adenovirus BACs
  • Production of low-passage virus stocks followed by amplification in 293 cells
  • Purification, titration of infectious units (IU) and QC
  • Gene expression quantification on mRNA level by qRT-PCR in a standard cell system and/ or
  • optionally on protein level by Western Blot
  • optionally transient inducible cell model generation
  • ADDITIONALLY needed: premade rAV expressing the transactivator

ScaleInfectious Units (IU)Time
Standard
≥ 1 X10E9
4-5 weeks
Medium
≥ 1 X10E10
5-6 weeks
Large*
≥ 1 X10E11*
7-9 weeks
X-Large *≥ 1 X10E12 *9-11 weeks

* Feasibility check needed

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