Dynamic expression switch

VariCHECK™ RNAi ON-Target Verification System

VariCHECKTM is an advanced gene engineering service that enables researchers to dynamically switch gene expression of an endogenous gene to a different, ectopically expressed gene of interest.

This helps examine cellular processes associated with disease-causing mutations in protein kinase domains, growth receptors, oncogenes and other key regulatory proteins. VariCHECKTM can be also designed to serve as a perfect RNAi built-in control to confirm ON-target phenotypes.

The VariCHECKTM system has been described in a publication of a the Journal of Biomolecular Screening - link.

Developmental process & fields of application

1. Identification of a highly efficient shRNA using RNAiONETM

2. Generation of a stable inducible knockdown cell pool using TET-inducible lentivirus technology

3. Parallel overexpression of any desired protein in the knockdown cell pool.

A)Stable inducible with lentivirus technology

B)Transient constitutive with adenovirus technology

Please contact us for more information and individual project discussions.