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pVal shRNA Validation Platform - RNAiONE - Your success gurarantyFields of ApplicationIdentification of optimal shRNA sequences
Project Statistics & Customer Satisfaction GuarantyOver the years SIRION BIOTECH has developed its base technology into an art: in 80% of recent projects SIRION BIOTECH managed to identify shRNAs with knockdown results of above 80%. Such experience makes it possible to issue warranties with its quotes. ![]() Principle
Available pVal platforms
Prices available on request Application ExampleScreening of 10 shRNA designs against the human TKTL-1 gene. A partial cDNA of TKTL-1 was cloned and 10 shRNA designs tested. The vector was transfected into HEK293 cells and the level of TKTL-1 mRNA quantified by qPCR (Table 1). Prevalidation Results Among the 10 sequences checked, only shRNA 2 achieved a knockdown superior to 90%, and 2 more shRNAs knocked down the gene to a satisfactory degree of 80% (shRNAs 6+7). ![]() Conformation of activity of prevalidated shRNAs in stable knockdown cell pools 3 knockdown cell pools in THP-1 cells were generated using the best 2 shRNA sequences (shRNA 2 and 7) and a NT shRNA sequence (control). The knockdown efficiency in the cell pools was determined on the mRNA level and with Western Blot. Relative quantification of target gene expression in stable cell pools by qRT-PCR and Western Blot. Both cell pools display a high degree of knockdown superior to 90% both on mRNA and protein level. Expression of the NT-shRNA (Control) had no effect on gene expression. ![]() |


