SALES CONTACT

For prices and further information please inquire:

T. +49 89 700 961 99 13
info@sirion-biotech.de
 

shRNA-AAV Vector Production Service

Fields of Application

  • Long term gene expression in vivo
  • Target validation and gene therapy

Service Comprises

  • Selection and screening for highly active shRNA sequences
  • Cloning of validated shRNA(s) into shRNA-AAV expression vectors
  • Verification of cloning success by DNA-Sequencing
  • Transfection of 293T cells with AAV expression vectors and helper plasmids
  • Purification of AAV vectors by affinity-chromatography
  • Production up to >5x1013 AAV particles

Benefits

  • From shRNA sequence to viral vectors with guaranteed efficacy in less than 3 month
  • Best vectors sytem for long term gene expression in vivo
  • Virus can be handled in S1 laboratories
  • Funcionality tested AAV vector preparations
  • Reliable results for in vivo target validation

Available shRNA-AAV Expression Vectors

RNA Polymerase III driven expression of target gene-specific shRNA from human U6 promoter

Vector Type

Promoter

Transgene

Product

Price

AAV2-9, rh10 

Human U6

shRNA

Small scale: >5x10^12 vp

On request

AAV2-9, rh10

Human U6

shRNA

Large scale: >1x10^13 vp

On request

Application Example

Long-term gene expression of a luciferase reporter gene in heart using a tissue-specific MLC promoter. After  i.v. injection of 1011 genomic particles of AAV.9-CMV-MLC1.5-Luc in vivo imaging with IVIS imaging system reveals heart-specific gene expression after 9 months