Fields of Application

• Mammalian expression and purification of proteins
• Functional gene analysis
• Target identification and validation
• Generation of reporter cell lines for screening
• Expression of fluorescent or luminescent proteins for in vivo imaging

Service Comprises

• Cloning the cDNA of Your Gene of Interest (GOI) in an expression vector
• Verification of cloning success by DNA-Sequencing
• Transfection/transduction of Your Cell Line with the resulting expression vector
• Generation of stable cell pools or clonal cell lines by antibiotic selection.
• Quantification of  expression of your GOI by qRT-PCR and/or Western Blot

Benefits

• Maximum flexibility in experimental design
• Homogenous GOI expression both on mRNA and protein level.
• GOI Expression adjustable by choice of promoter
• From receipt of your cDNA to delivery of a functionality tested stable cell pool in less than 6 weeks
• Funcionality tested frozen cells ready to use

Available Expression Systems

• Monocistronic or bicistronic expression of your GOI
• In all bicistronic vectors the expression of your GOI is coupled to that of the gene confering antibiotic resistance

Price for service is based on the customized options chosen and is available on request