SALES CONTACT

For prices and further information please inquire:

T. +49 89 700 961 99 13
info@sirion-biotech.de
 

Stable Gene Knockdown Cells

Fields of Application

  • Functional gene analysis
  • Target identification and validation
  • Xenotransplant models in oncology research

Service Comprises

  • Cloning of validated shRNA(s) into an U6-promoter driven shRNA expression vector
  • Verification of cloning success by DNA-Sequencing
  • Transfection/transduction of Your Cell Line with the resulting expression vector
  • Generation of stable cell pools or clonal cell lines by antibiotic selection.
  • Quantification of gene knockdown by qRT-PCR and/or Western Blot relative to control cells
  • Control cells expressing a Non-Target (NT) shRNA are included

Benefits

  • Reliable system for highest target gene knockdown
  • Guaranteed kockdown of 80% on mRNA
  • From shRNA sequence to delivery of a functionality tested stable knockdown cell pool in less than 6 weeks
  • Funcionality tested frozen cells ready to use

Available Expression Systems

RNA Polymerase III driven  expression of target gene-specific shRNA from human U6 promoter 

Vector System

Promoter

transgene

Vector type

U6-shRNA-Puro-LV

 human U6

shRNA

lentiviral

U6-shRNA-Puro-P

human U6

shRNA

plasmid

U6-shRNA-Neo-LV

 human U6

shRNA

lentiviral

U6-shRNA-Neo-P

human U6

shRNA

plasmid

Price for service is based on the customized options chosen and is available on request

Application Example

Constitutive knockdown of galectin 1 (Gal 1) in BeWo cells stably transduced with the U6-shRNA-Puro-LV vector. Quantification relative to NT-shRNA expressing control cells by qRT-PCR.

 

 

Cp Target       Gal1

Cp Ref.     PPIA

∆ Cp

∆ ∆ Cp

%Target expr

Non Target Ctrl

20,50

23,03

-2,53

0,00

100%

sh Gal1-124

26,32

22,63

3,69

6,22

1%