SALES CONTACT

For prices and further information please inquire:

T. +49 89 700 961 99 13
info@sirion-biotech.de

SERVICES

Cell Systems Adenovirus and AAV Service Customized Gene Knockdown Cells Customized Cells for Gene Overexpression Adenoviral Gene Expression Cells Stable Gene Expression Cells TET-ON/TET-OFF Inducible Stable Gene Expression Cells Genetically modified 3D microtissues shRNA Validation Service Cell Line Development

PRODUCTS

AV Cloning & Purification Transduction Enhancer Premade Adenovirus for gene knockdown and overexpression

Industrial Co-Development

Project Opportunities Immortalization of Primary Cells - Co-Development

TET-ON/TET-OFF Inducible Stable Gene Expression Cells

Fields of Application

Whenever temporal, spatial and quantitative control of transgene activity is an issue
If target gene knockdown has adverse effects on cell growth and/or vitality
Xenotransplant models in oncology research

Service Comprises

Cloning the cDNA of Your Gene of Interest (GOI) in an expression vector
Verification of cloning success by DNA-Sequencing
Transfection/transduction of Your Cell Line with the resulting expression vector
Generation of stable cell pools or clonal cell lines by antibiotic selection.
Quantification of inducible expression of your GOI by qRT-PCR and/or Western Blot

Benefits

Maximum flexibility in experimental design
Tight system with negligibly low background expression in the absence of Dox
Strong induction of GOI expression in the presence of Dox both on mRNA and protein level.
For direct analysis of On-Target-effects
Tunable Expression of Your GOI
From receipt of your cDNA to delivery of a functionality tested stable cell pool in less than 6 weeks
Funcionality tested frozen cells ready to use

Available Expression Systems

SIRION's Unique One-Vector system:
GOI and TET-responsive transactivator expressed from the same vector

Standard Two-Vector systems:
GOI and TET-responsive transactivator expressed from different vectors

Vector System	Type of regulation	Suitable size of cDNA	Vector type
One-vector-LV	TET-ON	up to 4 kb	lentiviral
One-vector-LV	TET-OFF	up to 4 kb	lentiviral
One-vector-P	TET-ON	up to 4 kb	plasmid
One-vector-P	TET-OFF	up to 4 kb	plasmid
Two-Vector-LV	TET-ON	> 4kb	lentiviral
Two-Vector-LV	TET-OFF	> 4kb	lentiviral
Two-Vector-P	TET-ON	> 4kb	plasmid
Two-Vector-P	TET-OFF	> 4kb	plasmid

Price for service is based on the customized options chosen and is available on request

Application Example

TET-inducible expression of a GOI encoding for a 120 kDa Protein in A549 cells stably transduced with the One-vector-LV TET-ON system of SIRION Biotech. Quantification was carried out by qRT-PCR and Western Blot.