Adenovirus Vectors for Gene Knockdown

Fields of Application

• For highly efficient, transient gene knockdown studies in vitro and in vivo

Service Comprises

• shRNA validation based on 5,10 or 15 sequences
• Cloning of validated shRNA(s) into adenovirus shuttle plasmids
• Verification of cloning success by DNA-Sequencing
• Generation of recombinant adenovirus BACs
• Production of low-passage virus stocks
• Amplification of the adenovirus vector in 293 cells
• Purification and Titration of infectious units (IU)
• Gene expression quantification on mRNA level by qRT-PCR and/or optionally on protein level by Western Blot

Benefits

• Patent pending BAC technology guarantees fast time lines resulting in less than 6 weeks from cloning to final virus preparation
• Fast and reliable time lines
• Highest product purity
• Quantitative shRNA delivery to and uniform expression in most cell types
• Functionality tested and guaranteed gene knockdown ≥ 80% on mRNA level 
• Degree of Knockdown adjustable by using different virus amounts
• Reproducible results by high efficient titration

Available Expression Systems

Different available promotors and marker genes guarantee for flexibility in vector design