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Serotype Ad19a/64

Overcome the drawbacks of commonly available Ad serotypes

Ad19a/64, the first openly accessible subtype D adenovirus. The vector is superior in its action to transduce human-derived cells (myoblasts and myotubes) much more efficiently when compared to Ad5.  It also possesses CAR independent binding properties, targeting a sialic acid-dependent receptors.

Ad19a/64 will help you

  • Target new cell types/tissue due to its unique receptor affinities.
  • Utilze its low pre-immunization rates within the human populationwhen developing Ad based vaccination.

Whenever you experience with Ad5

  • Limitations due to CAR dependant transduction or
  • Pre-existing anti-adenovirus immunity against in Ad5 in humans.

contact SIRION Biotech for its unique Ad19a/64-BAC-technology. SIRION Biotech is actively seeking strategic alliances for the development of commercial use of Ad19a/64 .The offered material is license-free for research only, any and all other uses require an explicit approval from Sirion Biotech GmbH.

Human Myoblasts

Transduction of myoblasts with Ad5-EGFP (Ad5) and Ad19a/64-EGFP (Ad19a). Primary myoblasts of human, ape, pig and mouse origin were transduced with 1250 VP/cell (open columns). 2500 VP/cell (shaded columns) and 12500 VP/cell (solid columns), respectively, and GFP expression was quantified after 48 hr. Each value represents the mean of transgene expression per well in relative light units (RLU) from five independent experiments +/- SD.

Human MT

Transduction of primary myotubes (MTs) with Ad5EGFP and Ad19a/64-EGFP. Ten-day-old myotubes were transduced with 1250 VP/cell (open columns). 2500 VP/cell (shaded columns) and 12500 VP/cell (solid columns), respectively. GFP expression was quantified after 48 hr. Each value represents the mean relative light units (RLU) of five independent transductions +/- SD.

About the recent renaming of Ad19a to Ad64, please refer to Zhou et al, Invest Ophthalmol Vis Sci.2012 May 14;53(6):2804-11. doi: 10.1167/iovs.12-9656.

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