Adeno-associated virus (AAV)
Custom AAV vectors for basic research and preclinical drug development
Due to their unique biology AAVs are considered the gold standard for in vivo expression and knockdown experiments and have emerged as leading vehicle for gene therapies.
SIRION BIOTECH masters FPLC-grade AAV production and engineering services, providing high quality products in as short as 4 weeks. The sophisticated platform allows the integration of virtually any desired expression construct within the vector’s cloning capacity. Batch sizes range from 1×1012 VG (vector genomes) for small explorative experiments all the way to 5×1014 VG to satisfy complete experimental cohorts.
Special Feature – AAV OPTIMIZATION FOR GENE THERAPY
SIRION BIOTECH offers manufacture of a range of AAV serotypes in combination with tissue specific promotors to further increase gene expression precision. Additional in-house features of the SIRION BIOTECH adeno-associated virus technology platform include surface modified particles for cell-specific transduction. Every project is personally communicated with our customers to ensure that the resulting particles are feasably designed and perfectly fit the final application.
Custom AAV
The success of AAV in vivo experiments is dependent on vector quality and titer. Equally important is the correct choice of serotype and promoter.
When choosing our custom AAV service, SIRION Biotech’s technical experts will assist you to identify the optimal setting for your specific application and engineer AAV vectors exactly to your specification.
Fields of Application
- Tissue specific long term gene overexpression and knockdown in vivo
SIRION benefits
- Personal expert consultation at all stages of the project
- Strong tissue targeting with a unique serotype and promoter portfolio
- AAV delivery in less than 2 months
- Highest quality standards and upscaling options to fit your experimental needs
Service Details
- Cloning of your cDNA/ shRNA into an expression vector with free choice of promoter
- Verification of cloning success by DNA-sequencing
- Small-scale test production with GO/ NO-GO decision
- GO: standard production
- Transfection of 293T cells with custom the expression vector and a serotype specific helper plasmids
- One or two-step purification and concentration inlcuding FPLC options
- Genomic titer determination, QC and Delivery
Contact us to draft your custom AAV project
AAV Configurator
Directly design your custom constructs at www.doulix.com and order SIRON AAV packaging service for full AAV particle automization.
