Engineered Adenovirus vectors for transient and quantitative gene expression or knockdown
SIRION BIOTECH`s patented BAC technology enables adenovirus construction in less than 5 weeks. The system complies with all standard safety criteria by using replication deficient E1/E3 deleted serotype Ad5 as basis. Virtually every desired gene or shRNA expression construct is possible, with a capacity of 7.5 kb.
Benefits
- Strong cell accessibility dividing and non-dividing cell
- Tight control of gene expression levels
- High titer productions for transgene delivery in vivo
- Inducible expression available to study toxic gene modulations
- No site specific mutagenesis due to episomal genome expression
In addition to standard Adeno applications, SIRION offers a range of further customization options including a choice of custom promoters and transduction enhancers for hard to transduce cell types. Any project is thoroughly discussed with our customers to ensure that the resulting particles are feasably designed and fit the final application.
Custom constitutive
Fields of Application
- Strong, tightly regulated and highly efficient transient gene overexpression and knockdown in vitro
- for primary cells and all hard to transfect cell types
- High titer production for in vivo applications available
Benefits
- Up to 4 genes from one vector through large packaging capacity (up to 7.5 kb)
- Large promoter and marker gene portfolio for max flexibility in vector design
- From cloning to viral delivery in less than 5 weeks – guaranteed with patented BAC technology
- Highest product purity
- Reproducible results through high-efficiency titration
- Tight control of gene expression levels by adapting virus concentration (MOI)
- knockdown efficiency of at least 80% on mRNA level when combining with our RNAiONE™ service
Service Details
- Cloning of customer cDNA (up to 7.5 kb)/ shRNA in SIRION`s adenovirus shuttle plasmid
- Verification of cloning success by DNA sequencing
- Generation of recombinant adenovirus BACs
- Production of low-passage virus stocks followed by amplification in 293 cells
- Purification, titration of infectious units (IU) and QC
- Gene expression quantification on mRNA level by qRT-PCR in a standard cell system and/ or
- optionally on protein level by Western Blot
- optionally transient cell model generation
Scale | Infectious Units (IU) | Time |
Standard | ≥ 1 X10E9 | 4-5 weeks |
Medium | ≥ 1 X10E10 | 5-6 weeks |
Large * | ≥ 1 X10E11 | 7-9 weeks |
X-Large* | ≥ 1 X10E12 | 9-11 weeks |
* Feasibility check needed/ also applicable forin vivo
Custom inducible
SIRION Biotech`s inducible expression platform is based on the latest 3G TET technology. This guarantees a highly sensitive doxycycline-inducible system without leakiness. Our inducible adenovirus platform is based on a TWO vector system.
Fields of Application
- Characterization of toxic gene modulations
- Strong, tightly regulated inducible gene overexpression in vitro
- Highly efficient inducible gene knockdown studies in vitro
- for primary cells and all hard to transfect cell types
Benefits
- Tightly controlled ON/ OFF switch by doxycycline
- From cloning to viral delivery in less than 5 weeks – guaranteed with patented BAC technology
- Highest product purity
- Reproducible results by high efficient titration
- Tight regulation of gene expression levels with different MOIs
- Large promoter and marker gene portfolio guarantees for flexibility in vector design
- knockdown efficiency of at least 80% on mRNA level when combining with our RNAiONE service
Service Details
- Cloning of your cDNA (up to 6 kb)/ shRNA in SIRION`s inducible adenovirus shuttle plasmid
- Verification of cloning success by DNA sequencing
- Generation of recombinant adenovirus BACs
- Production of low-passage virus stocks followed by amplification in 293 cells
- Purification, titration of infectious units (IU) and QC
- Gene expression quantification on mRNA level by qRT-PCR in a standard cell system and/ or
- optionally on protein level by Western Blot
- optionally transient inducible cell model generation
Scale | Infectious Units (IU) | Time |
Standard | ≥ 1 X10E9 | 4-5 weeks |
Medium | ≥ 1 X10E10 | 5-6 weeks |
Large* | ≥ 1 X10E11* | 7-9 weeks |
X-Large * | ≥ 1 X10E12 * | 9-11 weeks |
* Feasibility check needed
Contact us – to discover more
High titer for in vivo
Adenoviruses can be concentrated to high titers of 10^11 IU/ml making them an extremely efficient method of delivering shRNA or transgenes in vivo.
Additional benefits for in vivo use are the fast and strong onset of expression.
When using adenoviruses one must take into consideration that >90% of the virus particles will be absorbed by the liver and that there can be strong immunoreactions of the host organism.
BL-2 (S2) facilities are required when handling adenoviruses.
Fields of Application
- Efficient transgene/ shRNA expression in vivo
SIRION’s benefits
- Vector formulation for direct in vivo use
- Fast and strong expression onset
- highly concentrated [5 X10E10 – 1 X10E11 IU/ml]
- From cloning to viral delivery in less than 9 weeks – guaranteed with patented BAC technology
- Large promoter and marker gene portfolio guarantees for flexibility in vector design
- Highest product purity
- Up to 4 genes from one vector through large packaging capacity (up to 7.5 kb)
- Reproducible results due to standardized product quality
Service Details
- Cloning of your cDNA (up to 7.5 kb)/ shRNA in SIRION`s adenovirus shuttle plasmid
- Verification of cloning success by DNA sequencing
- Generation of recombinant adenovirus BACs
- Production of low passage virus stocks followed by amplification in 293 cells
- Upscaling in 293 cells to large/ X-large titer
- Specific purification via ultracentrifugation
- Titration of infectious units (IU) and QC
- Gene expression quantification on mRNA level by qRT-PCR in a standard cell system and/ or
- optionally on protein level by Western Blot
Scale | Infectious Units (IU) | Time |
Large * | ≥ 1 X10E11 * | 7-9 weeks |
X-Large * | ≥ 1 X10E12 * | 9-11 weeks |
Scale | Infectious Units (IU) | Time |
custom | ≥ 1 X10E14 * | xx weeks |
Highest amount manufactured at SIRION |
* Feasibility check needed
Premade AVs
- Ready to use human Adenovirus Type5 (dE1/E3)
- for transient constitutive overexpression and knockdown
- validated shRNAs with >80% knockdown guarantee on mRNA level
- functionality tested
- titer: 1X10E9 IU [1X10E10 IU/ml]
- delivery time: 1-2 weeks
- *please note shipping charges will be added
Control and marker genes
Product | Gene(s) | Cat# | Price* |
Ad-CMV-GFP | GFP | SB-P-AV-122-01 | 670,00 € |
Ad-CMV-ßGAL | BetaGal | SB-P-AV-014-01 | 670,00 € |
Ad-CMV-Luc2 | Luciferase | SB-P-AV-054-01 | 670,00 € |
Ad-CMV-Flp | Flp-Recombinase | SB-P-AV-051-01 | 670,00 € |
Ad-CMV-RFP | RFP | SB-P-AV-055-01 | 670,00 € |
Ad-PL | empty vector | SB-P-AV-123-01 | 570,00 € |
Ad-U6-NTshRNA | Non Target shRNA | SB-P-AV-146-01 | 620,00 € |
Ad-U6-NTshRNA-GFP | Non Target shRNA + GFP | SB-P-AV-146G-01 | 620,00 € |
iPSC Genes
Product | Gene(s) | Spezies | Ref.Seq | Cat# | Price* |
Ad-SFFV-OCT4 | OCT4 | human | NM_002701 | SB-P-AV-01-01 | 670,00 € |
Ad-SFFV-Sox2 | Sox2 | human | NM_003106 | SB-P-AV-02-01 | 670,00 € |
Ad-EF1A-myc | myc | human | NM_002467 | SB-P-AV-03-01 | 670,00 € |
Ad-EF1A-KLF4 | KLF4 | human | NM_004235 | SB-P-AV-04-01 | 670,00 € |
Ad_IPS_dTomato | OCT4, Sox2, KLF4 |
human | NM_002701, NM_003106, NM_004235 |
SB-P-AV-09-01 | 950,00 € |
Immortalizing Genes
Product | Gene(s) | Spezies | Ref.Seq | Cat# | Price* |
Ad-CMV-LTtsA58 | LT-tsA58 | SV40 | – | SB-P-AV-059-01 | 670,00 € |
Ad-CMV-E6-E7 | E6-E7 | HPV | – | SB-P-AV-062-01 | 670,00 € |
Ad-CMV-E2F1 | E2F1 | human | NM_005225 | SB-P-AV-063-01 | 670,00 € |
Ad_hTERT | hTERT | human | NM_198253 | SB-P-AV-064-0 | 670,00 € |
Other Genes
Product | Gene(s) | Spezies | Ref.Seq | Cat# | Price* |
Ad-CMV-GPR6 | GPR6 | human | NM_005284 | SB-P-AV-07-01 | 670,00 € |
Ad_DRD1 | DRD1 | human | NM_000794 | SB-P-AV-010-01 | 670,00 € |
Ad_DRD2 | DRD2 | human | NM_016574 | SB-P-AV-011-01 | 670,00 € |
Ad-CMV-Claudin1 | Claudin 1 | human | NM_021101 | SB-P-AV-015-01 | 670,00 € |
Ad-CMV-KCNK2 | KCNK2 | human | NM_014217 | SB-P-AV-032-01 | 670,00 € |
Ad-CMV-murf-1 | murf-1 | human | NM_032588 | SB-P-AV-041-01 | 670,00 € |
Ad-CMV-murf-2 | murf-2 | human | NM_033058 | SB-P-AV-042-01 | 670,00 € |
Ad-CMV-murf-3 | murf-3 | human | NM_032546 | SB-P-AV-043-01 | 670,00 € |
Ad-CMV-mLipG | LipG | mouse | NM_010720 | SB-P-AV-049-01 | 670,00 € |
Validated shRNA Adenovirus
(> 80% Knockdown on mRNA guaranteed)
Product | Gene | Spezies | Ref.Seq | Cat# | Price* |
Ad-sh-AKAP8 | AKAP8 | mouse | NM_019774 | SB-P-AV-019774-01 | 750,00 € |
Ad-sh-AKT1 | AKT1 | human | NM_005163 | SB-P-AV-005163-01 | 750,00 € |
Ad-sh-AKT2 | AKT2 | human | NM_001626 | SB-P-AV-001626-01 | 750,00 € |
Ad-sh-AKT3 | AKT3 | human | NM_005465 | SB-P-AV-005465-01 | 750,00 € |
Ad-sh-AurA | AurA | human | NM_198433 | SB-P-AV-198433-01 | 750,00 € |
Ad-sh-BAK | BAK | human | NM_001188 | SB-P-AV-001188-01 | 750,00 € |
Ad-sh-BAX | BAX | human | NM_004324 | SB-P-AV-004324-01 | 750,00 € |
Ad-sh-BCL2 | BCL2 | human | NM_000657 | SB-P-AV-000657-01 | 750,00 € |
Ad-sh-BCL-xL | BCL-xL | human | NM_138578 | SB-P-AV-138578-01 | 750,00 € |
Ad-sh-BCR | BCR | human | NM_004327 | SB-P-AV-004327-01 | 750,00 € |
Ad-sh-BECN1 | BECN1 | human | NM_003766 | SB-P-AV-003766-01 | 750,00 € |
Ad-sh-BECN1 | BECN1 | mouse | NM_003766 | SB-P-AV-003766-01 | 750,00 € |
Ad-sh-BID | BID | human | NM_197967 | SB-P-AV-197967-01 | 750,00 € |
Ad-sh-BIRC3 | BIRC3 | human | NM_001165 | SB-P-AV-001165-01 | 750,00 € |
Ad-sh-CASP3 | CASP3 | human | NM_032991 | SB-P-AV-032991-01 | 750,00 € |
Ad-sh-CASP3 | CASP3 | Sus scrofa | NM_214131 | SB-P-AV-214131-01 | 750,00 € |
Ad-sh-CASP6 | CASP6 | human | NM_001226 | SB-P-AV-001226-01 | 750,00 € |
Ad-sh-CASP7 | CASP7 | human | NM_033338 | SB-P-AV-033338-01 | 750,00 € |
Ad-sh-CASP8 | CASP8 | human | NM_001228 | SB-P-AV-001228-01 | 750,00 € |
Ad-sh-CASP9 | CASP9 | human | NM_001229 | SB-P-AV-001229-01 | 750,00 € |
Ad-sh-CAT | CAT | human | NM_001752 | SB-P-AV-001752-01 | 750,00 € |
Ad-sh-CD31 | CD31 | human | NM_000442 | SB-P-AV-000442-01 | 750,00 € |
Ad-sh-CD4 | CD4 | mouse | NM_013488 | SB-P-AV-013488-01 | 750,00 € |
Ad-sh-CD63 | CD63 | mouse | NM_007653 | SB-P-AV-007653-01 | 750,00 € |
Ad-sh-CDK5 | CDK5 | human | NM_004935 | SB-P-AV-004935-01 | 750,00 € |
Ad-sh-CDK6 | CDK6 | human | NM_001259 | SB-P-AV-001259-01 | 750,00 € |
Ad-sh-CDK9 | CDK9 | human | NM_001261 | SB-P-AV-001261-01 | 750,00 € |
Ad-sh-CEACAM1 | CEACAM 1 | human | NM_001712 | SB-P-AV-001712-01 | 750,00 € |
Ad-sh-Chip | Chip | human | NM_005861 | SB-P-AV-005861-01 | 750,00 € |
Ad-sh-DICER1 | DICER 1 | human | NM_030621 | SB-P-AV-030621-01 | 750,00 € |
Ad-sh-E-Cadherin | E-Cadherin | human | NM_004360 | SB-P-AV-004360-01 | 750,00 € |
Ad-sh-EGFR | EGFR | human | NM_005228 | SB-P-AV-005228-01 | 750,00 € |
Ad-sh-FADD | FADD | human | NM_003824 | SB-P-AV-003824-01 | 750,00 € |
Ad-sh-FAIM2 | FAIM2 | human | NM_012306 | SB-P-AV-012306-01 | 750,00 € |
Ad-sh-FGF2 | FGF2 | human | NM_002006 | SB-P-AV-002006-01 | 750,00 € |
Ad-sh-FOS | FOS | human | NM_005252 | SB-P-AV-005252-01 | 750,00 € |
Ad-sh-FOS | FOS | mouse | NM_010234 | SB-P-AV-010234-01 | 750,00 € |
Ad-sh-GAL1 | GAL1 | human | NM_002305 | SB-P-AV-002305-01 | 750,00 € |
Ad-sh-GAL3 | GAL3 | human | NM_002306 | SB-P-AV-002306-01 | 750,00 € |
Ad-sh-GNAS | GNAS | Sus scrofa | NM_214312 | SB-P-AV-214312-01 | 750,00 € |
Ad-sh-GSK3B | GSK3B | human | NM_002093 | SB-P-AV-002093-01 | 750,00 € |
Ad-sh-IER3 | IER3 | human | NM_003897 | SB-P-AV-003897-01 | 750,00 € |
Ad-sh-IKKa | IKKa | human | NM_001278 | SB-P-AV-001278-01 | 750,00 € |
Ad-sh-IKKb | IKKb | human | NM_001556 | SB-P-AV-001556-01 | 750,00 € |
Ad-sh-IKKg | IKKg | human | NM_003639 | SB-P-AV-003639-01 | 750,00 € |
Ad-sh-JAK1 | JAK1 | human | NM_004972 | SB-P-AV-004972-01 | 750,00 € |
Ad-sh-LEF1 | LEF1 | human | NM_016269 | SB-P-AV-016269-01 | 750,00 € |
Ad-sh-LMNA | LMNA | human | NM_002306 | SB-P-AV-002306-01 | 750,00 € |
Ad-sh-LMNA | LMNA | mouse | NM_001002011 | SB-P-AV-001002011-01 | 750,00 € |
Ad-sh-MDR1 | MDR1 | human | NM_000927 | SB-P-AV-000927-01 | 750,00 € |
Ad-sh-MET | MET | mouse | NM_008591 | SB-P-AV-008591-01 | 750,00 € |
Ad-sh-MET | MET | human | NM_008591 | SB-P-AV-008591-01 | 750,00 € |
Ad-sh-MMP9 | MMP9 | Sus scrofa | NM_001038004 | SB-P-AV-001038004-01 | 750,00 € |
Ad-sh-mTOR | mTOR | human | NM_004958 | SB-P-AV-004958-01 | 750,00 € |
Ad-sh-NOTCH1 | NOTCH 1 | human | NM_017617 | SB-P-AV-017617-01 | 750,00 € |
Ad-sh-P53(TP53) | P53 (TP53) | human | NM_000546 | SB-P-AV-000546-01 | 750,00 € |
Ad-sh-PIK3CA | PIK3CA | human | NM_006218 | SB-P-AV-006218-01 | 750,00 € |
Ad-sh-PKC-alpha | PKC-alpha | mouse | NM_011101 | SB-P-AV-011101-01 | 750,00 € |
Ad-sh-RB | RB | human | NM_000321 | SB-P-AV-000321-01 | 750,00 € |
Ad-sh-SIRT1 | SIRT 1 | human | NM_012238 | SB-P-AV-012238-01 | 750,00 € |
Ad-sh-SOD1 | SOD1 | human | NM_000454 | SB-P-AV-000454-01 | 750,00 € |
Ad-sh-STK16 | STK16 | human | NM_003691 | SB-P-AV-003691-01 | 750,00 € |
Ad-sh-Survivin | Survivin | human | NM_001168 | SB-P-AV-001168-01 | 750,00 € |
Ad-sh-TLR3 | TLR3 | rat | NM_198791 | SB-P-AV-198791-01 | 750,00 € |